Take control of your bioreactor experiments with Pharyx Microbioreactor Systems!

At Pharyx, we are developing systems to automate the study of microorganisms for basic research and bioprocess characterization and optimization. Through microfluidics and integration, large scale bioreactors can be miniaturized to the milliliter scale, enabling greatly reduced volumes in addition to automated control.

Run automated experiments in batch, fed-batch, chemostat, or turbidostat mode, with or without perfusion.

Working volume of 1mL saves precious reagents and simplifies continuous culture setups.

Combination of perfusion with chemostat or turbidostat mode enables control over the concentration of excreted products independent of cell dilution rate.
Microbioreactor Devices
  • 1mL working volume
  • Integrated pH, DO, and Optical Density sensors
  • Water plus 4 additional fluid feeds
  • Optional perfusion port (replaces a fluid feed)
  • Active mixing in seconds
  • kLa ~ 50-70 (1/hr)
  • Batch, Fed-batch, Chemostat, Turbidostat, Chemostat-perfustion, Turbidostat-perfusion
  • Customizable for different cell types

US Patent 7367550, Patents Pending



US Patent 7367550, Patents Pending
MCM-001 Microbioreactor control module
  • Independently operates one microbioreactor device
  • Fast setup with no individual tubing connections
  • Optional in-situ recalibration of sensors
  • Automated bioreactor setup
  • Automated fluid feeding
  • Temperature control (ambient – 45C)
  • pH control (5.5-8.5)
  • DO control (0% - 100% air saturation)
  • CO2 control option
  • Flow control (0.03-1.5 1/hr) dilution rate)


Patent Pending

MBS-004 Quad-mini Bioreactor Control Hub

Supports up to 4 multiplexed microbioreactor modules.
Space efficient: 33 cm (w) x 40 cm (d) x 33 cm (h) overall

Applications
  • Antibiotic PK/PD
  • Microbial antibiotic resistance frequency determination
  • Strain improvement, adaptation to inhibiting medium components
  • Small scale production of recombinant proteins under controlled conditions using perfusion-turbidostat
  • Synthetic biological circuit characterization under controlled, steady state conditions
  • Evolution Studies

Example of a chemical dosing profile every 6h over 24h using indicator dye

SS-001 System Software
  • Easy to use, intuitive software interface to Microbioreactor Control Modules.
  • Setup and run basic steady state experiments using the graphical user interface.
  • Powerful scripting interface to execute complex protocols.
    • set point sweeps
    • conditional set point adjustments
    • programmed induction
    • programmed feed composition variation
    • Serial batch experiments
  • Remote software and web access

Example Experiment

Growth rate vs. Temperature
  • E. coli turbidostat
  • 1mL working volume
  • M9 + glucose
  • Automated temperature steps
Turbidostat culture: dilution rate adjusts to maintain fixed optical density. Dilution rate equals maximum growth rate for the experimental conditions. Doubling time decreases as temperature increases from 31C to 37C.


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